. Author manuscript; available in PMC: 2014 Oct 22.

Published in final edited form as: Biochemistry. 2013 Oct 7;52(42):10.1021/bi401195r. doi: 10.1021/bi401195r

Table 1.

Rates of AlkD-catalyzed and spontaneous 7mG depurination from MeP-DNA

	k_st (× 10⁻³ s⁻¹) ^a	k_non (× 10⁻⁶ s⁻¹) ^a	k_st / k_non
M0	23.6 ± 1.1	1.0 ± 0.1	2.4 × 10⁴
M1	1.6 ± 0.4	1.8 ± 0.2	8.9 × 10²
M2	23.6 ± 1.1	1.0 ± 0.1	2.4 × 10⁴
M3	23.2 ± 1.2	1.1 ± 0.03	2.1 × 10⁴
M4	7.7 ± 0.5	1.1 ± 0.1	7.0 × 10³

Single-turnover (k_st) and non-enzymatic (k_non) rate constants for 7mG depurination from 25-mer oligonucleotides at 37°C, pH 7.5, and 150 mM ionic strength. M1-M4 each contained one MeP at the positions shown in Figure 2B. Data for M0 (no MeP) were taken from ref.³⁷ DNA sequences and kinetic data can be found in the Supplement.