Table 1.
The signal strength of the telomere and centromere probes after an 18-hour hybridization period at varying temperatures and conditions were rated as absent, poor, fair or strong
| Probes |
Goat Serum |
Formaldehyde |
FISH | ||||
|---|---|---|---|---|---|---|---|
| 4°C | RT | 37°C | 4°C | RT | 37°C | ||
| TelC |
Strong |
Strong |
Strong |
Strong |
Strong |
Strong |
Strong |
| TelG |
Poor HB |
Fair |
Strong |
Poor HB |
Fair |
Strong |
Strong |
| CENPB Box |
Absent |
Poor |
Strong |
Fair |
Strong |
Strong |
Strong |
| Cent | Absent | Absent | Absent | Absent | Absent | Absent | Strong |
High background was indicated as, HB, frequently found accompanying that signal only at the specified conditions. FISH indicates the traditional FISH protocol was used.