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. 2013 Oct 8;6:42. doi: 10.1186/1755-8166-6-42

Table 1.

The signal strength of the telomere and centromere probes after an 18-hour hybridization period at varying temperatures and conditions were rated as absent, poor, fair or strong

Probes Goat Serum
Formaldehyde
FISH
4°C RT 37°C 4°C RT 37°C
TelC
Strong
Strong
Strong
Strong
Strong
Strong
Strong
TelG
Poor HB
Fair
Strong
Poor HB
Fair
Strong
Strong
CENPB Box
Absent
Poor
Strong
Fair
Strong
Strong
Strong
Cent Absent Absent Absent Absent Absent Absent Strong

High background was indicated as, HB, frequently found accompanying that signal only at the specified conditions. FISH indicates the traditional FISH protocol was used.