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. Author manuscript; available in PMC: 2014 Mar 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2013 Feb 20;52(12):10.1002/anie.201210079. doi: 10.1002/anie.201210079

Figure 2.

Figure 2

(A) Electrophoretic analysis of copper-catalyzed ligation reactions between linearized (BamH I), platinated pUC19 plasmid DNA and Alexa Fluor 488-alkyne with reaction conditions indicated for each incubation (for details see Supporting Information). The top gel shows Alexa Fluor fluorescence monitored at 520 nm. Note the increase in band intensities and mobility shift with increasing platinum content. The latter band shift is caused by accumulation of positive charge on the plasmid. The bottom panel depicts the same gel stained with ethidium bromide. Saturation of DNA binding sites by Alexa Fluor-tagged platinum–acridine causes inefficient staining by ethidium at rb = 0.2. (B) CD spectra recorded in the ICD region for unmodified DNA (dotted trace), DNA modified with compound 2 (dashed trace), and DNA modified with compound 2 after ligation with Alexa Fluor 488-alkyne (solid trace).