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Intracellular distribution of compound 2 in a NCI-H460 cell in interphase. Cells were co-stained with nuclear (Hoechst 33342) and mitochondrial (MitoTracker Deep Red) dyes. The nucleolus, which is readily identified in the bright-field image and as a nuclear region unstained by Hoechst dye, appears as an area of high fluorescent intensity in the Alexa Fluor 488 and merged channel images.
