Table 2.
Mitochondrial shape variation and density of the matrix
| |
Shape |
Status of matrix |
|||
|---|---|---|---|---|---|
| Rod | Branching | Fragmented | Clear | Dense (Lipoid) | |
|
Control |
28 (93.3%) |
0 (0.0%) |
2 (6.7%) |
30 (100.0%) |
0 (0.0%) |
|
1% CSE |
25 (80.6%) |
6 (19.4%) |
0 (0.0%) |
31 (100.0%) |
0 (0.0%) |
|
2.5% CSE |
31 (96.9%) |
0 (0.0%) |
1 (3.1%) |
28 (87.5%) |
4 (12.5%) |
|
10% CSE |
24 (70.6%) |
2 (5.9%) |
8 (23.5%) |
23 (67.6%) |
11 (32.4%) |
|
12.5% CSE |
24 (69.6%) |
3 (8.6%) |
8 (22.9%) |
29 (82.9%) |
6 (17.1%) |
|
-10% CSE |
19 (63.3%) |
9 (30.0%) |
1 (3.1%) |
22 (73.3%) |
8 (26.7%) |
| *** | *** | ||||
***Estimated by chi-square test (p < 0.001).
BEAS-2B cells were exposed to various concentrations of cigarette smoke extract (CSE) for 6 months and subjected to EM analysis. BEAS-2B cells are exposed to: 0% CSE (control), 1% CSE, 2.5% CSE, 10% CSE, 12.5% CSE and -10% CSE (depletion). In total of 30–35 mitochondria were analyzed per experimental condition. Significance was determined by chi-square test.