Table 3.
Frequencies and percentages of number of cristae and number of cristae divided by length
| |
Number of cristae per mitochondrion per section |
FRQ/L** |
||||
|---|---|---|---|---|---|---|
| 0 | < 3 | 3-6 | >6 | Mean ± SD | Mean ± SD | |
|
Control |
0 (0.0%) |
0 (0.0%) |
2 (6.7%) |
28 (93.3%) |
2.9 ± 0.3 |
2.2 ± 0.8 |
|
1% CSE |
0 (0.0%) |
2 (6.5%) |
9 (29.0%) |
20 (64.5%) |
2.6 ± 0.6 |
1.9 ± 0.6 |
|
2.5% CSE |
4 (12.5%) |
4 (12.5%) |
8 (25%) |
16 (50%) |
2.1 ± 1.1* |
1.7 ± 1.0* |
|
10% CSE |
4 (11.8%) |
5 (14.7%) |
19 (55.9%) |
6 (17.6%) |
1.8 ± 0.9* |
1.3 ± 0.8* |
|
12.5% CSE |
0 (0.0%) |
5 (14.3%) |
18 (51.4%) |
12 (34.3%) |
2.2 ± 0.7* |
1.6 ± 0.6* |
|
-10% CSE |
1 (3.3%) |
4 (13.3%) |
12 (40%) |
13 (43.3%) |
2.2 ± 0.8* |
1.6 ± 0.7* |
| *** | **** | **** | ||||
*p < 0.05 pairwise comparison to 0% CSE (Control).
**Frequency divided by length.
***Estimated by chi-square test (p < 0.001).
****Estimated by One-Way ANOVA test (p < 0.001).
BEAS-2B cells were exposed to various concentrations of cigarette smoke extract (CSE) for 6 months and subjected to EM analysis. Representative images of BEAS-2B cells exposed to: 0% CSE (control), 1% CSE, 2.5% CSE, 10% CSE, 12.5% CSE and -10% CSE are shown in Figure 1. In total of 30–35 mitochondria were analyzed per experimental condition. Significance was determined by chi-square test and One-Way ANOVA.