Table 2.
Calibration curves, LOD, LOQ, Precision and Repeatability for ten compounds in Shen-Fu decoction
| Compound name | Calibration curve | r | Linear range(ng·mL-1) | LOD(ng·mL-1) | LOQ(ng·mL-1) | Intra-day(n=6) | Inter-day(n=6) | Repeatability(n=5) |
|---|---|---|---|---|---|---|---|---|
| Ginsenoside-Rb1 |
Y=11.04X+181.44 |
0.9930 |
3.90~125.00 |
0.97 |
3.00 |
3.44 |
4.11 |
6.51 |
| Ginsenoside-Rb2 |
Y=31.68X+246.74 |
0.9882 |
3.90~125.00 |
0.97 |
3.00 |
2.23 |
3.55 |
7.01 |
| Ginsenoside-Rc |
Y=20.13X+25.79 |
0.9921 |
3.90~125.00 |
1.25 |
3.00 |
2.02 |
3.92 |
4.21 |
| Ginsenoside-Rd |
Y=13.60X+69.00 |
0.9993 |
3.90~125.00 |
0.75 |
1.95 |
4.18 |
4.71 |
3.16 |
| Ginsenoside-Re |
Y=18.52X+136.68 |
0.9973 |
3.90~125.00 |
0.48 |
1.95 |
3.34 |
5.06 |
4.67 |
| Ginsenoside-Rf |
Y=37.66X+473.14 |
0.9952 |
3.90~125.00 |
0.97 |
3.00 |
2.05 |
3.12 |
5.03 |
| Ginsenoside-Rg1 |
Y=52.38X+109.20 |
0.9994 |
3.90~125.00 |
0.48 |
1.50 |
2.29 |
2.49 |
4.12 |
| Aconitine |
Y=6193.52X-80.34 |
0.9996 |
0.03~1.25 |
0.01 |
0.04 |
2.76 |
3.51 |
4.45 |
| Mesaconitine |
Y=3617.22X-63.02 |
0.9939 |
0.03~1.25 |
0.01 |
0.04 |
3.97 |
3.28 |
4.96 |
| Hypaconitine | Y=1207.82X+180.44 | 0.9960 | 0.19~6.24 | 0.01 | 0.04 | 2.55 | 2.42 | 4.81 |