Skip to main content
. 2013 Oct 7;44(1):96. doi: 10.1186/1297-9716-44-96

Figure 3.

Figure 3

Phylogenetic analysis of rabbit hemorrhagic disease virus (RHDV) isolates and RHDV2 isolates identified in Italian hares and rabbits. For the phylogenetic analysis of the VP60 sequences, the neighbor-joining method was applied based on the Kimura 2-parameters using the software package MEGA5 [20]. The European brown hare syndrome virus (EBHSV) strain BS89 (GenBank accession number: X98002) was used as an outgroup to root the tree. Bootstrap probability values above 70% for 1000 replicate runs are indicated at the nodes. Twenty selected sequences representing previously described RHDV genogroups (G1-G6, [18]), four sequences of RCV-like isolates, and the sequences of the RHDV2 isolates identified in France in 2010 (strain 10–32) and in Italy (strains Ud11 and Tn12-1) were included. The GenBank accession numbers of these strains are indicated. The RHDV sequences from rabbits isolated before spring 2011 (accession numbers: KC595270 and KC345614) belong to genogroup 5, whereas those from rabbits and hares collected after this period (accession numbers: KC345611, KC345612, KC345613, and JX106023) belong to the genogroup RHDV2.