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miR-497 targeted cyclin E1. (A) The miR-497 binding site in cyclin E1 3′-UTR, located 247–254 bp upstream of the cyclin E1 3′-UTR. (B) The relative luciferase of activity in miR-497 group and NC group (*P < 0.05). (C) Western blot analysis of cyclin E1 protein levels in miR-497-overexpressing and NC cells.
