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. 2013 Nov 20;2013:953162. doi: 10.1155/2013/953162

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Copyright © 2013 L. Liu and R. T. F. Cheung.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Schematic overview of the experimental procedures in a rat model of transient MCAO. Two groups are included in the experiment. The horizontal line represents time. A single i.p. injection of the vehicle and melatonin was given 30 minutes before transient MCAO in control and MEA groups, respectively. Whilst the rats were under anesthesia, sham and real EA were given daily 6 days before transient MCAO in the sham EA group and EA group, respectively. All the rats were killed 24 hours after transient MCAO for determination of brain infarct volume, cerebral edema ration and tissue processing.