Table 1.
Kinetic fit parameters and calculated rates of ground state recovery and photoinduced electron transfer rates for data averaged over the range 430 to 465 nm, after excitation at 375 nm, of wild-type, V108L, V108A, W106A MR, and wild-type PETNR with and without the addition of NAD(P)H4 coenzyme
| τ1 (ps) relative amplitude | τ2 (ps) relative amplitude | τ3 (ps) relative amplitude | kS0 (ps−1) | kET (ps−1) | |
|---|---|---|---|---|---|
| wt MR | 0.26 ± 0.05 47 ± 12% | 3.3 ± 0.2 33 ± 1% | 16.6 ± 0.7 19 ± 1% | 0.23 ± 0.01 | 0.10 ± 0.03 |
| wt MR -NADH4 | 0.26 ± 0.03 63 ± 11% | 3.2 ± 0.2 27 ± 1% | 19.9 ± 1.3 10 ± 1% | 0.33 ± 0.02 | |
| V108L | - | 3.9 ± 0.1 58 ± 1% | 22.3 ± 0.8 42 ± 1% | 0.09 ± 0.01 | 0.07 ± 0.01 |
| V108L-NADH4 | 0.24 ± 0.03 48 ± 10% | 4.5 ± 0.1 33 ± 1% | 26.2 ± 0.9 19 ± 1% | 0.15 ± 0.01 | |
| V108A | - | 3.0 ± 0.1 70 ± 2% | 17.0 ± 1.0 30 ± 2% | 0.14 ± 0.01 | 0.11 ± 0.05 |
| V108A-NADH4 | 0.47 ± 0.08 53 ± 8% | 4.4 ± 0.4 40 ± 2% | 27.3 ± 8.5 8 ± 2% | 0.25 ± 0.05 | |
| W106A | - | 2.5 ± 0.3 81 ± 8% | 8.1 ± 1.9 19 ± 9% | 0.28 ± 0.06 | 0.25 ± 0.08 |
| W106A - NADH4 | 0.34 ± 0.05 59 ± 9% | 4.0 ± 0.4 43 ± 1% | - | 0.53 ± 0.04 | |
| PETNR | - | 1.8 ± 0.4 40 ± 4% | 22.4 ± 1.9 60 ± 2% | 0.07 ± 0.01 | |
| PETNR - NADH4 | 0.77 ± 0.11 79 ± 8% | - | 8.5 ± 1.2 21 ± 2% | 0.41 ± 0.06 | 0.34 ± 0.07 |
| PETNR - NADPH4 | 0.63 ± 0.08 84 ± 8% | - | 7.8 ± 1.1 16 ± 2% | 0.56 ± 0.09 | 0.49 ± 0.09 |
Errors quoted on the lifetimes and relative amplitudes are those from the fitting procedure, it should be noted that the time resolution of the system is on the order of 200 fs, and this is the value used for error propagation to the rate values if the error on the fit is smaller than this.