Skip to main content
. 2013 Dec 3;105(11):2586–2597. doi: 10.1016/j.bpj.2013.10.025

Figure 2.

Figure 2

1H-15N HSQC spectrum of 0.6 mM 15N/13C-labeled 5P12-RANTES-E66S (50 mM KH2PO4, 0.02% NaN3, pH 3.8, 5% D2O) recorded at 37°C on a 600-MHz spectrometer. Backbone amide resonances are labeled with the assignments. The N-terminal glutamine Q0 undergoes a spontaneous cyclization to pyroglutamate (mechanism shown in the inset) resulting in the appearance of Q0 resonance and a shift of the G1 resonance from G1f to G1c (marked by arrow) corresponding to the free and the cyclic N-terminus, respectively.