Figure 3.

Secondary ¹³C^α (a) and ¹³C^β (b) chemical shifts analysis of RANTES-E66S dimer (37°C), 5P12-RANTES-E66S (37°C), and 5P12-RANTES-E66S in the presence of 1% FC-12 (at 25 and 60°C). Secondary structure elements according to the crystal structure PDB:1EQT and the amino acid sequence of RANTES-E66S as well as of 5P12-RANTES-E66S are drawn at the top. (Note that the sequence used in PDB:1EQT differs from human wt-RANTES by the mutations S1M, P2G, and L19M.) The extraordinary large secondary shifts for Q0 are the result of the pyroglutamate formation.