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. Author manuscript; available in PMC: 2013 Dec 6.
Published in final edited form as: J Cancer Sci Ther. 2012 Jul 26;4(7):214–222.

Figure 3.

Figure 3

The cells stained with ABCG2-specific aptamer ABCG2/A12 or ABCG2-specific mAb can form mammospheres. A. ABCG2/A12 did not recognize the differentiated breast cancer MCF-7 cells: left section, cells were stained with DAPI; middle section, with FITC-labeled ABCG2/A12; right section, merge of the previous two sections. B. ABCG2/A12 recognizes the ABCG2-expressing BCS cells in mammospheres derived from MCF-7. C. ABCG2/A12 and ABCG2-specific mAb 5D3 recognized the same BCS cells in mammospheres: left section, cells were stained with DAPI; second section, with FITC-labeled ABCG2/A12; third section, with APC-conjugated ABCG2-specific mAb 5D3; right section, merge of the previous three sections. D. Short time trypsin digestion completely abolished the binding of ABCG2/A12 and ABCG2-specific mAb 5D3 to BCS cells in mammospheres. E. BCS cells in mammospheres derived from MCF-7 were co-stained with BCS/A35 and ABCG2-specific mAb 5D3: left section, cells were stained with DAPI; second section, with FITC-labeled BCS/A35; third section, with APC-conjugated human ABCG2-specific mAb 5D3; right section, merge of the previous three sections. F. Short time trypsin digestion completely abolished the binding of BCS/A35 and ABCG2-specific mAb 5D3 to BCS cells in mammospheres. G. FITC-ABCG2/A12 negative (#12−) cells (4,000), sorted out by FACS from mammospheres derived from MCF-7, can barely form mammospheres. H. FITC-ABCG2/A12 positive (#12+) cells (4,000), sorted out by FACS from mammospheres derived from MCF-7, can form mammospheres. I. APC-conjugated ABCG2-specific mAb 5D3 negative (5D3−) cells (4,000) can barely form mammospheres. J. APC-conjugated ABCG2-specific mAb 5D3 positive (5D3+) cells (4,000) can form mammospheres. K. Numbers of mammospheres formed from above four cell populations.