Table 2.
miRNA | Sequence | Length | Stem-loop length | MFE [kcalmol-1] | Read count mature strand | Read count star strand |
---|---|---|---|---|---|---|
smb107.2 |
CAAGGAUGGGAUGCUCAGAGAA |
22 |
88 |
−69.3 |
13,315 |
398 (9) |
smb107.3 |
CAAGGAUGGGAUGGUCAGAGAA |
22 |
88 |
−64.1 |
3,322 |
398 (9) |
smb123 |
CAGUCGGCCAAAGUGCUGGACC |
22 |
89 |
−64.1 |
451 |
154 (9) |
smb203 |
CUUUGUAUCCCGGAUCCUGAUA |
22 |
87 |
−46.6 |
1,087 |
389 (9) |
smb215 |
GAGGAUGCUGAUCAUUCACUGG |
22 |
87 |
−80.6 |
85 |
34 (8) |
smb295 |
UCAGAGACCAGACGCAGAGGCU |
22 |
90 |
−40.6 |
12,543 |
160 (9) |
smb297 |
UCAGUGGCAGAAGCUGGGAACU |
22 |
87 |
−63.5 |
965 |
60 (8) |
smb313 |
UCGAACUUUCAGGAAUAGUAUC |
22 |
87 |
−54.8 |
2,707 |
1475 (9) |
siRNA |
Sequence |
Length |
Stem-loop length |
MFE [kcalmol-1] |
Read count mature strand |
Read count star strand |
smb21 |
AAUUUGAACGUUGCCAUCUAUC |
22 |
87 |
−72.5 |
123 |
9 (7) |
smb41 |
ACCUGCAGCAUUUGGCGCCUGA |
22 |
84 |
−77.9 |
299 |
18 (7) |
smb51 |
ACUUAGAACUCUCCUACGAGGG |
22 |
88 |
−83.1 |
510 |
275 (8) |
smb79 |
AGUUGGACCAGACCAGUUGGUC |
22 |
87 |
−71.7 |
489 |
319 (9) |
smb83 |
AUCACUCCACAAAGGGAUUUG |
21 |
87 |
−65.5 |
217 |
7 (5) |
smb101 |
CAACGAGAUUGGCCUUCUGUGC |
22 |
87 |
−82.9 |
5,234 |
412 (9) |
smb163 |
CGGGACUCGAUUCGGAGGGUGC |
22 |
88 |
−63.6 |
2,015 |
660 (9) |
smb271 |
UAGAAUGUAGUCGUCAUCUUGC |
22 |
88 |
−68.9 |
1,044 |
29 (9) |
smb303 |
UCCGCCGUGCAACUGUCGCAAC |
22 |
88 |
−80.2 |
207 |
107 (9) |
smb359 |
UGAUGUACAUCGAUUGAUCGAC |
22 |
86 |
−63.8 |
646 |
23 (9) |
smb365 |
UGCCAACGUGAUUUGCAACUCC |
22 |
84 |
−68.1 |
333 |
75 (7) |
smb379 |
UGGACUUGGAAAGCUUCUCUGC |
22 |
86 |
−76.7 |
2,505 |
2 (2) |
smb427 | UUUGUCCAGUGUACCUGCGCU | 21 | 85 | −73 | 728 | 50 (8) |
Read counts for guide and passenger strands were derived by pooling counts over conditions. The number of experimental treatments that identified the passenger strand is indicated in brackets. MFE = Minimum Free Energy of precursor.