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. 2013 Oct 12;14:704. doi: 10.1186/1471-2164-14-704

Table 2.

Set of 21 smRNAs (8 miRNAs and 13 siRNAs) that were independently identified over 9 experimental treatments and matched all criteria for smRNA identification

miRNA Sequence Length Stem-loop length MFE [kcalmol-1] Read count mature strand Read count star strand
smb107.2
CAAGGAUGGGAUGCUCAGAGAA
22
88
−69.3
13,315
398 (9)
smb107.3
CAAGGAUGGGAUGGUCAGAGAA
22
88
−64.1
3,322
398 (9)
smb123
CAGUCGGCCAAAGUGCUGGACC
22
89
−64.1
451
154 (9)
smb203
CUUUGUAUCCCGGAUCCUGAUA
22
87
−46.6
1,087
389 (9)
smb215
GAGGAUGCUGAUCAUUCACUGG
22
87
−80.6
85
34 (8)
smb295
UCAGAGACCAGACGCAGAGGCU
22
90
−40.6
12,543
160 (9)
smb297
UCAGUGGCAGAAGCUGGGAACU
22
87
−63.5
965
60 (8)
smb313
UCGAACUUUCAGGAAUAGUAUC
22
87
−54.8
2,707
1475 (9)
siRNA
Sequence
Length
Stem-loop length
MFE [kcalmol-1]
Read count mature strand
Read count star strand
smb21
AAUUUGAACGUUGCCAUCUAUC
22
87
−72.5
123
9 (7)
smb41
ACCUGCAGCAUUUGGCGCCUGA
22
84
−77.9
299
18 (7)
smb51
ACUUAGAACUCUCCUACGAGGG
22
88
−83.1
510
275 (8)
smb79
AGUUGGACCAGACCAGUUGGUC
22
87
−71.7
489
319 (9)
smb83
AUCACUCCACAAAGGGAUUUG
21
87
−65.5
217
7 (5)
smb101
CAACGAGAUUGGCCUUCUGUGC
22
87
−82.9
5,234
412 (9)
smb163
CGGGACUCGAUUCGGAGGGUGC
22
88
−63.6
2,015
660 (9)
smb271
UAGAAUGUAGUCGUCAUCUUGC
22
88
−68.9
1,044
29 (9)
smb303
UCCGCCGUGCAACUGUCGCAAC
22
88
−80.2
207
107 (9)
smb359
UGAUGUACAUCGAUUGAUCGAC
22
86
−63.8
646
23 (9)
smb365
UGCCAACGUGAUUUGCAACUCC
22
84
−68.1
333
75 (7)
smb379
UGGACUUGGAAAGCUUCUCUGC
22
86
−76.7
2,505
2 (2)
smb427 UUUGUCCAGUGUACCUGCGCU 21 85 −73 728 50 (8)

Read counts for guide and passenger strands were derived by pooling counts over conditions. The number of experimental treatments that identified the passenger strand is indicated in brackets. MFE = Minimum Free Energy of precursor.