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. 2013 Oct 17;14(1):108. doi: 10.1186/1465-9921-14-108

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Copyright © 2013 Winden et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pecam-1 expression in RAGE TG compared to controls. Lungs from control (Co, A) and RAGE TG (B) mice were qualitatively immunostained for Pecam-1, revealing no appreciable differences. Images were at 100X original magnification. Quantitative RT-PCR (C) and immunoblotting (D) for Pecam-1 using standardized aliquots of total lung mRNA or protein revealed markedly decreased Pecam-1 expression in RAGE TG mouse lung compared to controls. *Statistical difference (P ≤ 0.05) with at least three replicates per group.