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. 2013 Dec 4;8(12):e82485. doi: 10.1371/journal.pone.0082485

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© 2013 Sagaram et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Sequence of MtDef4 and its variants. The γ-core motif is indicated in larger font. RGFRRR sequence of MtDef4 is indicated in bold and the conserved amino acids are listed underneath with highly conserved amino acids on the top followed by less conserved ones. RGFR sequence which closely resembles the RXLR motif of the fungal and oomycete effectors is italicized.

B. Images showing the inhibition of F. graminearum PH-1 conidial germination and hyphal growth at different concentrations of MtDef4 or its variants. Images were taken after 16 hours of incubation of conidia with defensins. Bar = 50 μm.

C. Quantitative assessment of the in vitro antifungal activity of MtDef4 or its variants at 4 days after incubation of PH-1 conidia with defensins. Values are means of thee replications. Error bars indicate standard deviations.

D. Images showing the growth of PH-1 strain after 6 days in the presence of MtDef4 or its variants.