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. 2013 Dec 4;8(12):e82485. doi: 10.1371/journal.pone.0082485

Figure 10. DyLight 550-labeled MtDef4 and MtDef4RGFRRR/RGAARR but not MtDef4RGFRRR/AAAARR and MtDef4RGFRRR/RGFRAA enter F.

Figure 10

graminearum cytoplasm.

F. graminearum conidia were incubated with indicated concentrations of DyLight 550-labeled proteins and confocal fluorescence images were taken at various time intervals for up to 6 h.

A. Within 15 min, DyL-MtDef4, DyL-MtDef4RGFRRR/RGAARR and DyL-MtDef4RGFRRR/RGFRAA bound to the surface of conidia whereas DyL-MtDef4RGFRRR/AAAARR did not.

B. At 2 h, DyL-MtDef4 and DyL-MtDef4RGFRRR/RGAARR bound to the surface of germ tubes but DyL-MtDef4RGFRRR/AAAARR and DyL-MtDef4RGFRRR/RGFRAA did not.

C. DyL-MtDef4 entered selective hyphae by 4 h.

D. By 6 h, DyL-MtDef4RGFRRR/RGAARR entered hyphae but not all hyphae were affected.

E. DyL-MtDef4RGFRRR/AAAARR did not enter the hyphae even after 6 h.

F. DyL-MtDef4RGFRRR/RGFRAA bound to the surface of conidial cells but did not bind to hyphal surface.

Scale Bar = 10 µm.