graminearum cytoplasm.
F. graminearum conidia were incubated with indicated concentrations of DyLight 550-labeled proteins and confocal fluorescence images were taken at various time intervals for up to 6 h.
A. Within 15 min, DyL-MtDef4, DyL-MtDef4RGFRRR/RGAARR and DyL-MtDef4RGFRRR/RGFRAA bound to the surface of conidia whereas DyL-MtDef4RGFRRR/AAAARR did not.
B. At 2 h, DyL-MtDef4 and DyL-MtDef4RGFRRR/RGAARR bound to the surface of germ tubes but DyL-MtDef4RGFRRR/AAAARR and DyL-MtDef4RGFRRR/RGFRAA did not.
C. DyL-MtDef4 entered selective hyphae by 4 h.
D. By 6 h, DyL-MtDef4RGFRRR/RGAARR entered hyphae but not all hyphae were affected.
E. DyL-MtDef4RGFRRR/AAAARR did not enter the hyphae even after 6 h.
F. DyL-MtDef4RGFRRR/RGFRAA bound to the surface of conidial cells but did not bind to hyphal surface.
Scale Bar = 10 µm.