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Evaluation of processing speed and segmentation accuracy. (a) Processing time of CellProfiler, ImageJ/Fiji, and cellXpress in segmenting Kc167, HT29 and HeLa image datasets. (b) Segmentation accuracy of CellProfiler, ImageJ/Fiji, and cellXpress. (k = total number of image frames, *** = P<0.001, n.s. = P>0.05; two-sided t-test).
