FIGURE 1.
Multiple assays for assessment of chemokine signaling. Concentration-response data for G protein signaling (left column; cAMP signal demonstrating inhibition of forskolin-stimulated cAMP by Gi/o), βarr recruitment (middle column), and receptor internalization (right column) for CCR1 (A), CCR5 (B), CCR10 (C), CXCR1 (D), CXCR2 (E), and CXCR3 (F) are shown. Shown are means ± S.E. from at least three experiments and fits with logistic dose-response functions with Hill coefficient equal to 1 (Table 2). Error bars represent S.E.