FIGURE 5.

H₂O₂ regulates oogenesis and choriogenesis. A and B, H₂O₂ injection. dsMal- and dsRpDuox-treated females were injected with 5 nmol of H₂O₂ into the hemocoel 24 h ABM, and the number of eggs laid (A) and the first instar nymph eclosion rates (B) were evaluated. C and D, catalase knockdown. 6 days before the blood meal, adult females were injected with dsMal, dsDuox, dsCat, or dsDuox plus dsCat. C, catalase silencing was evaluated by qPCR in whole ovary extracts. D and E, the number of eggs laid (D) and eclosion ratios (E) were calculated individually for five females in each condition. In all graphic data shown are the mean ± S.E., and the asterisks indicate significantly different values between the conditions (B, p = 0.0205; D, p = 0.0032; Student's t test).