FIGURE 2.

The synthetic glycan G1M3 inhibits glycan-dependent and independent calreticulin-substrate interactions. A–D, representative BLI sensorgrams depicting inhibition of the interactions between 0.5 μm mCRT(WT) and IgY-EN (A), deglycosylated IgY-EN (B), β2M (C), and LLO(d123) (D) following preincubation of calreticulin with varying concentrations of the glycan G1M3. The calculated IC₅₀ values and data replicates are detailed in Table 1. E and F, representative ITC profiles for mCRT(WT) (E) and mCRT(Y92A) (F) binding G1M3. The figures show representative titration curve fits of two independent experiments. G and H, representative DSF curves (of two independent experiments) measuring the change in the thermostability of mCRT(WT) (G) and mCRT(Y92A) (H) in the presence or absence of G1M3.