Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Grx2c reduces CRMP2 in a dithiol reaction mechanism. Incubation of reduced (red) Grx2c with oxidized (ox) CRMP2 did not lead to a complex between the proteins when analyzed by non-reducing SDS-PAGE. Only incubation with a Grx2c mutant lacking the second (resolving) cysteinyl residue in the CXXC (C40S) active site led to the formation of a mixed disulfide (see arrowhead). In addition, wild-type Trx1 was able to reduce the protein in vitro and did not form a stable complex with CRMP2.
