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. 2013 Oct 23;288(49):35138–35148. doi: 10.1074/jbc.M113.486795

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Colocalization of ectopic overexpressed Sox9 and E6-AP in COS7 cells and chondrocytic cell line HCS-2/8 cells. GFP-fused SOX9 and Halo-tagged or FLAG-tagged E6-AP were transiently overexpressed in COS7 cells (A) or HCS-2/8 cells (B) either individually or together. Halo-tagged E6-AP was detected with TMR ligand, and FLAG-tagged E6-AP was detected with anti-FLAG antibody. A, in COS7 cells, SOX9 shows a slightly punctuated subnuclear localization, whereas E6-AP is seen in the nucleus, perinucleus, and cytoplasm when it is expressed alone. In some photos, z axis images of the horizontal and vertical line in the photos are shown. B, in HCS-2/8 cells, SOX9 and E6-AP localize in nucleus and mostly colocalized together.