FIGURE 1.

MyT1 sequence information. A, schematic of the mouse MyT1 protein. ZFs 1–7 are indicated, as are the limits of the double ZF constructs used in this study. B, sequence alignment of MyT1 ZFs as well as the domains from NZF1 and NZF3 for which structures are available (rat NZF1 (rNZF1), PDB ID 1PXE; human NZF3 (hNZF3), PDB ID 2CS8). Arrows and asterisks indicate DNA-binding residues and zinc-coordinating residues, respectively. mMyT1, mouse MyT1. C, RARE DNA sequence with numbering used in this work. Box indicates the protein-binding motif.