FIGURE 7.

NMR analysis of the interaction of F5F6 with DNA. A, portion of the ¹⁵N HSQC of ¹⁵N-labeled F5F6 (right) in the absence (gray) and presence (black) of 1 molar eq of RARE DNA and comparison with the same data for F4F5 (left). B, SPR data (average from n = 3 replicates) showing the ability of mutant oligonucleotides to compete with chip-bound RARE DNA for the binding of F5F6 in the wild-type complex. All mutants (black bars) except C11G (right gray bar) compete significantly less well than wild-type RARE (left gray bar), indicating that these bases most likely are contacted by F5F6. C, imino region of a one-dimensional ¹H NMR spectrum of RARE DNA in the presence of F5F6 (solid line) and comparison with the same region of a spectrum of the F4F5-RARE complex (dotted line).