Yeast two-hybrid pairwise confirmation of hits from the cDNA library screen. Yeast transformants harboring pLexA plasmid (baits, indicated above the image) and the pGAD-HA plasmid (preys, indicated in the left column) were grown on SD-Leu-Trp (growth indicates the presence of each indicated plasmid) as well as SD-Leu-Trp-His (growth indicates synthesis of the HIS3 enzyme, suggesting an interaction between the expressed proteins), SD-Leu-Trp-His+3AT (3AT is a competitive inhibitor of the HIS3 gene product and is used to titrate the lowest amount of HIS3 expression required for growth on media lacking histidine) or SD-Leu-Trp-His-Ade (the most stringent selection media) plates. A, both of the tested proteins interact with FDX2. B, all of the tested proteins interact equally with FDX3. C, all of the tested proteins (uncharacterized protein GI: 159470998) interact with FDX4. UBQ1 interacts the most strongly resulting in robust growth, although the uncharacterized protein (GI: 159485179) results in weak but detectible growth. D, all of the tested proteins interact with FDX5 (uncharacterized protein GI: 159475534).