Yeast two-hybrid bridging assay for HYDA1 (bait) and the maturation factors HYDEF and HYDG (preys). Yeast transformants harboring the indicated pLexA plasmid (pLexA-p53 or pLexA-C-HYDA1), pGAD-HA plasmids (empty, pGAD-HA-HYDEF, or pGAD-HA-HYDG), and bridging plasmid (pY3H-HYDEF or pY3H-HYDG) were grown on SD-Leu-Trp-Ura and SD-Leu-Trp-Ura-His. Increased growth is observed for yeast harboring HYDA1, HYDEF and HYDG.