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. 2013 Oct 7;288(49):35192–35209. doi: 10.1074/jbc.M113.483727

FIGURE 5.

FIGURE 5.

Yeast two-hybrid bridging assay for HYDA1 (bait) and the maturation factors HYDEF and HYDG (preys). Yeast transformants harboring the indicated pLexA plasmid (pLexA-p53 or pLexA-C-HYDA1), pGAD-HA plasmids (empty, pGAD-HA-HYDEF, or pGAD-HA-HYDG), and bridging plasmid (pY3H-HYDEF or pY3H-HYDG) were grown on SD-Leu-Trp-Ura and SD-Leu-Trp-Ura-His. Increased growth is observed for yeast harboring HYDA1, HYDEF and HYDG.