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The oxygenation reaction of 3HB6H. The first step is formation of C4a-peroxy anion. The second step is a parallel reaction of protonation of a peroxy group to form C4a-hydroperoxyflavin and an uncoupling path for hydrogen peroxide elimination. The third step is a reaction of hydroxylation. The fourth step is dehydration of water from C4a-hydroxyflavin to return to the oxidized flavin, of which the reaction is very fast, preventing the detection of C4a-hydroxyflavin.
