FIGURE 2.

cAMP production and CPAR activation. Lysates were prepared from accumbens MSNs (10 DIV), and intracellular cAMP was measured by ELISA. A, cAMP concentrations normalized to vehicle after treatment with AMPA (50 μm, 1 min) and/or NASPM (200 μm) and inhibitors (APV, 10 μm; CdCl₂, 50 μm; TTX, 500 nm) as indicated. B, cAMP concentrations after treatment with NMDA (50 μm, 1 min) and/or SKF38393 (10 μm, 1 min) and SCH23390 (10 μm) as indicated. Concentrations are fmol/well with n = 6 wells/test condition. Inhibitors/antagonists were added 30 min before treatment. Data are represented as the mean ± S.E. normalized to vehicle treatment and analyzed using one-way ANOVA followed by Fisher's post hoc tests. N.S., not significant.