FIGURE 5.

Multiple sequence alignment of the strawberry Fra a 1E, Fra a 2, and Fra a 3 proteins and other related Bet v 1/START proteins. The secondary structural elements correspond to the Fra a 1E (black) and LIPR-10.2B (blue) structures. The L5 loop has been highlighted in yellow. Residues oriented toward the cavity and residues involved in catechin binding (for Fra a 3) are indicated by dark green boxes and light green triangles, respectively. Positions showing sequence variations, which are either important for catechin binding (cyan) or facing the cavity (black stars), are also indicated. The sequence alignment was performed using ClustalW (EBI server), and the figure was generated by ESPript (72).