FIGURE 2.

Effects of PI3K isoform-selective inhibitors on viability of RAW264.7. Undifferentiated RAW264.7 cells were treated with inhibitors (100 pm to 10 μm, or 1 nm to 100 μm for LY294002) or vehicle for 24 h, after which viability was assessed using an MTT assay. Data are expressed as a percentage of values for vehicle-treated control samples. Dashed lines indicate 100%. Viability was diminished only by PIK75 at concentrations of 1 and 10 μm and LY294002 at 100 μm. Data are mean ± S.E., n = 4 independent experiments, except for IC87114 and LY294002, where n = 3. Differences were assessed using one-way ANOVA and Tukey's multiple comparisons test. * indicates p < 0.05 compared with vehicle.