FIGURE 4.

LcrH is required for YopD association with 30 S ribosomal particles. A–E, immunoblot analysis of hydrophobic interaction chromatography fractions collected during isolation of ribosomal particles from Y. enterocolitica strains lcrH (A), lcrH (plcrH) (B), yopD (C), yopD (pyopD) (D), and yscM1/yscM2 (E). Each strain was grown in the presence of either 5 mm CaCl₂ (secretion non-permissive) or 5 mm EGTA (secretion permissive). Polyclonal antisera raised against class II factors YopD, LcrH, and YscM1 as well as monoclonal antibodies against ribosomal protein S3 were used to probe collected fractions. FT = flow-through fractions. Average percentages of YopD or YscM1 in the cleared lysate that are associated with either 30 or 70 S ribosomal particles are listed in Table 1. Images and data analyses are representative examples from three independent experiments.