TABLE 1.

Association of YopD and YscM1 with ribosomal 30 S and 70 S particles

Straina	Fractionb	YopDc				YscM1c
		CaCl2d	Pe	EGTAd	P	CaCl2	P	EGTA	P
Wild type	30 S	12.3 (0.8)f		15.1 (2.7)		6.77 (3.9)		5.7 (6.9)
	70 S	0.5 (0.4)		1.7 (1.5)		2.40 (2.4)		10.1 (17.)
lcrH	30 S	4.6 (3.2)	<0.05	0.5 (2.2)	0.004	9.78 (11.2)	0.7	1.8 (4.1)	0.5
	70 S	0.6 (0.9)	0.9	0.5 (2.4)	0.3	17.3 (19.)	0.3	21.0 (11.)	0.4
lcrH (plcrH)	30 S	17.0 (6.6)	0.7	14.3 (1.2)	0.7	1.90 (1.5)	0.2	0.7 (2.2)	0.3
	70 S	3.2 (4.3)	0.4	0.2 (0.2)	0.2	2.45 (3.9)	1.0	8.2 (7.8)	0.9
yopD	30 S	NDg		ND		1.35 (0.4)	0.1	3.9 (3.7)	0.7
	70 S	ND		ND		2.71 (1.6)	0.9	23.0 (21.)	0.5
yopD (pyopD)	30 S	12.1 (1.0)	0.9	16.9 (2.6)	0.086	1.11 (1.0)	0.1	1.0 (1.1)	0.3
	70 S	0 (0.3)	0.9	0.5 (1.2)	0.3	1.90 (0.7)	0.8	0.8 (0.8)	0.4
yscM1/yscM2	30 S	13.7 (9.4)	0.8	15.4 (2.6)	0.9	ND		ND
	70 S	4.5 (6.2)	0.4	0.00 (6.0)	0.6	ND		ND

^a Identifies Y. enterocolitica strain used to generate ribosomal fractions, including complementing plasmids in parentheses.

^b Fractions comprising the 70 S and 30 S ribosomal particles were isolated by hydrophobic interaction chromatography.

^c Fractions were subjected to quantitative immunoblotting using chemiluminescence to detect YopD or YscM1.

^d Cells were grown in TSB with 5 mm CaCl₂ (secretion non-permissive condition or with 5 mm EGTA (secretion-permissive).

^e Statistical significance of YopD or YscM1 present in the indicated fractions compared to wild-type samples was evaluated with the unpaired Student's t test and p values recorded.

^f Abundance of YopD or YscM1 was measured with quantitative immunoblotting in three independent determinations, averaged with S.D. calculated in parentheses.

^g ND, not detected.