TABLE 2.

Inhibition of yopQ mRNA translation in the presence of H6-YopD/LcrH (added to Yersinia or E. coli ribosomes at a concentration of 13.3 μm) and GST-YscM

Factora	Y. enterocolitica ribosomesb				E. coli ribosomesc
	yopQd	Pe	nptf	Pg	yopQ	P	npt	P
GST-YscM1 (13.3 μm)	0.68	0.023	0.97	0.906	1.00	0.725	0.93	0.786
GST (13.3 μm)	1.09		0.95		1.04		1.25
GST-YscM1 (26.6 μm)	0.37	0.005	0.83	0.357	0.95	0.227	1.02	0.544
GST (26.6 μm)	1.04		0.96		1.00		1.08
GST-YscM1 (39.9 μm)	0.33	0.013	1.12	0.511	0.88	0.918	1.29	0.190
GST (39.9 μm)	0.98		0.98		0.85		1.58

^a Purified GST-YscM1 or GST were added to Yersinia or E. coli ribosomes at indicated concentrations.

^b Ribosomes were isolated via hydrophobic interaction chromatography from Y. enterocolitica W22703 grown in TSB at 37 °C with 5 mm EGTA and added at a concentration of 13.3 μm.

^c Ribosomes were isolated via hydrophobic interaction chromatography from E. coli BL21 (DE3) grown in LB at 37 °C and added at a concentration of 13.3 μm.

^d Translation of yopQ was measured with quantitative immunoblotting using LI-COR in three or more independent determinations, normalized to control samples and averaged.

^e Statistical significance of YopQ abundance between GST-YscM1 vs. GST samples was evaluated with the paired Student's t test and p values recorded.

^f Translation of npt was measured with quantitative immunoblotting using LI-COR in three or more independent determinations, normalized to control samples, and averaged.

^g Statistical significance of Npt abundance between GST-YscM1 vs. GST samples was evaluated with the paired Student's t test and p values recorded.