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p16 modulates the expression of miR-141 and miR-146b-5p in a cell cycle-independent manner. A, confluent cells were starved for 24 h in serum-free media, and then the cell cycle was analyzed by flow cytometry. The numbers in the boxes indicate the proportion of cells in each phase of the cell cycle. PI, propidium iodide. B, total RNA was purified from the indicated cells and was amplified by qRT-PCR using specific primers for the indicated genes. Error bars represent means ± S.D.
