FIGURE 5.

p16 positively regulates the expression of miR-141 and miR-146b-5p through the Sp1 transcription factor. A, nucleotide sequences of the miR-141 and miR-146b-5p promoters, −39 to +9 nucleotides and −93 to −26 nucleotides, respectively, relative to the putative transcription start site. Highlighted are the various overlapped Sp1-binding sites in the miR-141 and miR-146b-5p promoters. B and D, whole cell lysates were prepared from the indicated cells and used for immunoblotting using the indicated antibodies. C and E, EMSAs were performed using nuclear extracts from the indicated cells and biotin-labeled Sp1 consensus binding site of the miR-146b-5p promoter. Oligos, oligonucleotides.