FIGURE 5.

Thermal stabilities of WT and mutant mouse decorin. A, unfolding transitions obtained by differential scanning calorimetry at a protein concentration of 3 mg/ml in PBS. The melting temperatures derived from the peak maxima are 50 (WT) and 52 °C (Q61N), respectively. B, unfolding transitions obtained by differential scanning fluorimetry at a protein concentration of 0.181 mg/ml in PBS. The melting temperatures derived from the inflection points of the curves are 49 (R151E), 50 (WT and Y51A/R52A/Q54A), and 51 °C (Q61N and Y130N), respectively. Shown is a representative of two independent experiments carried out in triplicate. A.U., arbitrary units.