TABLE 1.

Molecular masses of mouse decorin and its mutants as determined by SEC-MALS

The relative errors of the experimentally determined masses are <5%.

Protein	Calculated molecular mass	N-Linked glycosylation sites	Peak elution volume	Experimental mass of glycoproteina	Experimental mass of polypeptide fractionb
	kDa		ml	kDa	kDa
WT	36.2	4	14.5	83.5	64.4
Y51A/R52A/Q54A	36.2	4	14.1	85.3	65.3
Q61N	36.2	5	15.5	52.1	37.6
Y130N	36.2	5	15.1	51.5	36.7
R151E	36.2	4	14.1	88.4	68.4

^a Derived from the refractive index and light scattering signals.

^b Derived from the absorbance, refractive index, and light scattering signals (26).