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Removing the LIM domains of migfilin does not result in increased migfilin association with filamin. A, GFP-migfilin constructs were transfected into CHO cells and immunoprecipitated using a GFP antibody. Endogenous FLNa co-precipitation was detected by Western blotting and quantified (B). inp, 5% input material. IP, immunoprecipitated. n = 4. *, p = 0.01; ****, p < 0.0001 using Student's t test. IB, immunoblot; ns, nonspecific.
