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. 2013 Oct 28;288(49):35604–35616. doi: 10.1074/jbc.M113.483016

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Migfilin localization in kindlin-2 knockdown fibroblasts. NIH3T3 fibroblasts were infected with lentivirus containing control (SC, scrambled) shRNA or kindlin-2 (K2) shRNA. A, these cell lysates were probed for endogenous kindlin-2 using FLNa as a loading control. B, using lysate of CHO cells transfected with FLAG-kindlin-1 and lysates of scramble (SC) or kindlin-2 knockdown keratinocyte (KT) as positive controls, SC or K2 knockdown 3T3 lysates were probed for endogenous kindlin-1. C, scramble control cells were plated on fibronectin and transfected with GFP-migfilin full-length (WT), then fixed and stained for endogenous vinculin. D and E, kindlin-2 knockdown 3T3 cells were transfected with GFP-migfilin WT or migfilin ΔLIM2,3 (F) and stained for endogenous vinculin or F-actin. Scale bar = 20 μm; inset scale bar, 5 μm.