FIGURE 8.

Kindlin-2 is required for migfilin recruitment to and release from focal adhesions. A, example still taken from FRAP movies of NIH3T3 cells stably expressing control or kindlin-2 shRNA transfected with GFP-migfilin. Cells were imaged on a confocal microscope, regions of interest were bleached using 488 nm laser at maximum power for 2 s. B, cumulative FRAP recovery traces from control and kindlin-2 shRNA cells (n = 10 cells per condition, and >2 adhesions per cell). Fluorescence recovery is normalized to pre-bleach levels and corrected for any fading of fluorophores over time. C, bar graphs of (top) τ_½ recovery times and (bottom) mobile fractions of migfilin-GFP in control or kindlin-2 knockdown cells. **, p < 0.001.