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. 2013 Dec 4;8(12):e82383. doi: 10.1371/journal.pone.0082383

Figure 4. Pre-analytical handling.

Figure 4

A. Ten aliquots of serum samples from 5 volunteers were subjected to up to ten cycles of thawing and freezing. Each line represents the ELISA results measuring sMFAP4 (U/ml) in aliquots from one single volunteer. The CV% per volunteer ranges from 7.1% to 12.6%. B. Eight serum samples from a single, healthy volunteer were left at RT for varying time intervals before centrifugation and separation (1, 5, 24, 48 or 192 hours). The sMFAP4 measurement for the one-hour sample is used as the index sample corresponding to 100%. C. Ten serum samples were withdrawn from 5 healthy volunteers using different tube types (serum tubes or serum-gel tubes) and varying temperature and time intervals before centrifugation and separation as indicated below the graph. Each line represents the ELISA results from one single volunteer using preparation number 1 as the index sample corresponding to 100%. The CV% per volunteer for all ten measurements ranges from 3.5% to 9.6%.