Figure 2.

Autoradiogram of an UV-crosslink assay performed with IRES domain V labeled probes and an extract prepared from HeLa cells. Proteins were resolved in a 10% SDS-PAGE. Domain V of FMDV IRES was used as positive control (lane DV FMDV). Lanes DV CVB3 _(Wt) and DV CVB3 _(S3) demonstrate the RNA–protein interaction pattern of CVB3 wild-type and Sabin3-like IRES domains V. Equal amounts of total proteins (10 μg) were loaded per well. The molecular weight (MW) of each complex was determined by comparing to a concurrently loaded MW marker: Prestained SDS-page standards broad range (Biorad).