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. 2013 Dec 4;8(12):e82449. doi: 10.1371/journal.pone.0082449

Figure 4. KLF6 rendered SCs more susceptible to apoptotic stress.

Figure 4

A. KLF6 mRNA expression analyzed by real-time PCR in RSC96 cells overexpressing KLF6 (KLF6-vector) and RSC96 cells stably transfected with the empty vector (Vector). *p<0.05 vs. Vector (t-test). B. Western blot of KLF6 expression in RSC96 cells. C. RSC96 cells stably transfected with control vector control or overexpressing KLF6 were treated with 100 ng/ml TNFα for 48 hours and then subjected to TUNEL staining. Representative images are shown along with the quantification of 5 randomly selected fields. Original magnification, 200×; *p<0.05 vs. the TNF+vector group (t-test). D. RSC96 cells were treated with 16 μg/ml cisplatin for 8 hours and then subjected to TUNEL staining. Representative images are shown along with the quantification of 5 randomly selected fields. Original magnification, 200×; *p<0.05 vs. the Vector + Cisplatin group (t-test).