Figure 3. SS transmission is blocked by Ni²⁺.

A, recording from NS_trans during a stimulus train protocol to NS_cis (upper panel) as in Fig. 1A but in the presence of extracellular Ni²⁺ (0.1 mm). B, addition of Ni²⁺ to the bath resulted in a moderate reduction in the inward current evoked by a depolarization beyond HVA calcium channel threshold (+10 mV). C, family of Ca²⁺ currents evoked by pulse depolarizations in the presence of extracellular Ni²⁺. D, relationship of steady-state Ca²⁺ current amplitude to voltage plotted for control NS_cis recordings and in the presence of Ni²⁺ (n= 8).