Figure 7. The effect of Congo red (CR) on the microtubules of pom2-4 and than/+ root tips.

Treatment for 6 h on pom2-4 (a, c) and than/+ (b, d) root tips, at single CLSM sections (a, b) or maximum projections (c, d). In both mutants, cortical microtubules appear mainly transverse in the meristematic zone (a, b). In the transition zone (arrowheads in c, d), protodermal cells exhibit transverse microtubules, while in the elongation zone (arrows in c, d) cortical microtubules are randomly oriented. Scale bars, 20 μm.