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. 2013 Dec 6;4:423. doi: 10.3389/fimmu.2013.00423

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Copyright © 2013 Chaumeil and Skok.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamics of Tcra association with H3K4me3- enriched active loci in WT and RAG1-deficient DN2/3 and DP cells. DNA FISH was performed using fluorescent BAC probes for Tcra, Igh, and the H3K4me3-enriched active loci, images were recorded on a Leica SP5 confocal and distances were measured in 3-dimension using Image J software. Association was defined as a distance between the two centers of mass under 1 μm in DN2/3 (A) and DP (B) cells.