Table 1.
Physicochemical properties of uncoated and lipid-coated SPIONs in different buffer solutions at pH 7.4
| Buffer system | Particle concentration (mg/mL) |
Particle size (nm) |
Zeta potential (mV) |
||
|---|---|---|---|---|---|
| Uncoated SPIONs | Lipid-coated SPIONs | Uncoated SPIONs | Lipid-coated SPIONs | ||
| |
1.0 |
520.0 ± 45.4 |
651.6 ± 25.3 |
-32.4 ± 1.0 |
-11.9 ± 1.4 |
| Citrate, pH 7.4 |
0.24 |
286.6 ± 25.4 |
460.3 ± 15.4 |
-40.7 ± 1.4 |
-15.6 ± 1.4 |
| |
0.02 |
80.0 ± 1.7* |
179.3 ± 35.0** |
-47.1 ± 2.6* |
-19.1 ± 1.3** |
| |
1.0 |
1860.0 ± 180.9a |
2422.0 ± 223.5a |
-11.2 ± 1.0 |
-4.5 ± 0.9 |
| HBSS, pH 7.4 |
0.24 |
1255.0 ± 35.2a |
1560.0 ± 135.2a |
-12.3 ± 1.1 |
-5.5 ± 1.0 |
| |
0.02 |
580.0 ± 8.5 |
193.5 ± 32.6** |
-23.3 ± 0.8 |
-7.4 ± 1.4 |
| |
1.0 |
2800.0 ± 320.4a |
2990.0 ± 412.5a |
-10.3 ± 0.5 |
-2.2 ± 0.6 |
| PBS, pH 7.4 |
0.24 |
2214.0 ± 45.3a |
2500.0 ± 245.3a |
-10.8 ± 1.0 |
-3.4 ± 1.1 |
| 0.02 | 931.0 ± 4.5 | 229.9 ± 12.42** | -22.5 ± 0.8 | -5.2 ± 1.6 | |
Data are represented as mean ± SD (n = 4).
aValue outside qualification range of Zetasizer Nano-ZS.
*Significantly different from uncoated control SPIONs (p < 0.05).
**Significantly different from lipid-coated SPIONs (p < 0.05).