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Real-time RT-PCR analysis of ATF4 and CHOP mRNAs in the DP-12-ATF4-16 clone. Total RNA was isolated from CHO cells after 72 h of cultivation. The mRNA levels were standardized to β-actin in CHO cells and were then compared to the expression level in the original host cell line at 72 h. Error bars represent the standard error of the mean (n = 3; single determination from each of three independent culture samples). **P < 0.001, two sided unpaired t test
